Not known Factual Statements About flow cytometers
Not known Factual Statements About flow cytometers
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Information-enriched characterization of mobile populations. Morphological details from illustrations or photos can increase on the richness of flow cytometry information. For instance, the figure demonstrates an if not typical apoptosis assay utilizing Annexin V and PI, introducing mobile imaging to characterize cells in Every inhabitants to reveal morphologically distinct characteristics. These insights couldn't have already been obtained from multiplex staining alone.
As samples are obtained on the Attune CytPix Flow Cytometer, the high-velocity brightfield digicam captures and stores photos of detected situations at a rate up to 6,000 illustrations or photos for each second, depending upon the flow level and image dimension. For greater flexibility, Attune Cytometric Computer software lets you modify the image capture frequency as required and choose a certain gate for graphic capture, if required.
Combine acoustic focusing technological innovation with superior-parameter Evaluation for versatile spectral unmixing and traditional workflows.
Find new insights into your experimental data by visualizing populations employing mixed fluorescence and morphology details. Our software interface wizards enable downsampling, concatenation (merged data files) and dimensionality equipment which might be developed in the software program for an intuitive, streamlined info Assessment practical experience.
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Below, a seasoned person has gated singlets confidently. Following assessing the manual singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of over 4% aggregates.
Pair your Attune flow cytometer with the autosampler for amplified workflow effectiveness, and include robotic integration for optimum throughput.
Steady impression high-quality even at substantial throughput Acoustic focusing and a higher-speed digicam Mix to impression these T cells constantly at lower or high flow rates. Conveniently regulate concentrate and digital camera options to fulfill experimental needs.
Early indications of this contamination had to begin with been dismissed as particles. Backgating to find out new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also helps you to use morphological functions to find out exciting subpopulations that would not be evident from flow cytometry knowledge on your own.
End users can perspective occasion photographs in genuine-time to substantiate sample good quality, improve protocols, and history the morphology of each and every cell populace discovered for Assessment. Imaging capture also Gains from acoustic concentrating, which really helps to placement the cells to ensure that a sharp, centered graphic is received.
AI-driven automatic picture Assessment Using the Attune Cytometric Software program interprets function attributes into distinctive morphology parameters which might be combined with normal fluorescence and scatter parameters to reveal extremely reproducible population variations and exceptional subsets.
Software Be aware: Automatic graphic Evaluation minimizes person-to-consumer variability in flow cytometry gating approaches
Leverage the strength of Highly developed engineering and innovation to accelerate your discoveries. Have faith in inside the Attune Flow Cytometer family members to provide the reliability, speed, and accuracy that established your function apart.
No clean/no lyse leukocyte analysis with imaging. Cells ended up acquired from 24-hour-previous blood by dilution in one mM EDTA ( Expert UX/UI enables consumers to coach AI styles for expanded purposes. (A) The “Practice Design” characteristic gives annotation resources and genuine-time product teaching accuracy.
At the guts of each Attune Flow Cytometer lies our revolutionary acoustic focusing technologies, which utilizes sound waves to meticulously align cells inside the flow mobile. This modern technique surpasses conventional hydrodynamic methods, causing speedier processing speeds, Excellent facts high-quality, and elevated sample throughput.
The Attune Xenith cytometer is actually a higher-parameter spectral analyzer, offering both of those spectral unmixing and conventional flow abilities for complete and flexible mobile Examination.
Agilent Price Promise guarantees you at the very least a decade of instrument use with the day you purchase. Otherwise, We're going to credit rating you Along with the residual value of the technique toward an upgraded model.
Listed here, we display that by gating methods utilizing impression-primarily based quantitative parameters can distinguish interacting cells https://www.4shared.com/office/UY9BjuVJku/Flow_Cytometer_A_Revolutionary.html from coincident occasions to much more precisely assess interacting cells.